Effect of gelatinous spongy scaffold containing nano-hydroxyapatite on the induction of odontogenic activity of dental pulp stem cells

Tissue engineering methods may be applied for the repair or regeneration of damaged teeth by inducing cell proliferation and differentiation in endodontic regeneration. One main factors tissue is scaffold used. An ideal should able to facilitate predictable development Thus, requires consideration composition functionality suited biological applications. The objective this study was assess morphological, physico-chemical, properties a new gelatin (Gel)-nano-hydroxyapatite (nHA)-based containing nHA prepared freeze-drying method. Conventional techniques, including scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FT-IR), X-ray diffraction analysis (XRD), dynamic light scattering (DLS) were used evaluate physico-chemical fabricated Gel-nHA sponge scaffold. Biological examination survival dental pulp stem cells (DPSCs) evaluated MTT assay ALP activity respectively. produced had spongy that all functional groups hydroxyapatite present sponge. In studies, viability grown on no different change from control group days 2nd, 4th, 6th. Besides, after 14 cultured Gel-nHA, alkaline phosphatase (ALP) showed significant increment. summary, revealed favorable effects odontogenic activity, implying potential future application